SYBR Green qPCR primers for detecting rat Tle5 (TLE family member 5, transcriptional modulator, also called R-esp1) gene expression by real-time PCR.
Rat Tle5 qPCR Primer Set – TLE family member 5, transcriptional modulator
Also known as: Aes, Grg, Grg-5
About this gene: Tle5 is predicted to enable identical protein binding activity and transcription corepressor activity. It is predicted to be involved in multiple processes, including negative regulation of canonical Wnt signaling pathway; positive regulation of anoikis; and regulation of gene expression. It is predicted to act upstream of or within regulation of DNA-templated transcription; regulation of growth; and skeletal system development.
Gene details: NCBI Gene ID: 29466; UniProt: P63003; Chromosome location: 7q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.