SYBR Green qPCR primers for detecting rat S1pr2 (sphingosine-1-phosphate receptor 2, also called S1P2) gene expression by real-time PCR.
Rat S1pr2 qPCR Primer Set – sphingosine-1-phosphate receptor 2
Also known as: Edg5, GPCR18, Gpcr13, H218, snGPCR18
About this gene: S1pr2 enables G protein-coupled peptide receptor activity. It acts upstream of or within G protein-coupled receptor signaling pathway; negative regulation of smooth muscle cell migration; and negative regulation of vascular associated smooth muscle cell proliferation. Predicted to be active in multiple cellular components, including glutamatergic synapse; plasma membrane; and presynapse.
Gene details: NCBI Gene ID: 29415; UniProt: P47752; Chromosome location: 8q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.