SYBR Green qPCR primers for detecting rat Edem1 (ER degradation enhancing alpha-mannosidase like protein 1) gene expression by real-time PCR.
Rat Edem1 qPCR Primer Set – ER degradation enhancing alpha-mannosidase like protein 1
Also known as: RGD1563633
About this gene: Edem1 is predicted to enable mannosyl-oligosaccharide 1,2-alpha-mannosidase activity and misfolded protein binding activity. It is predicted to be involved in multiple processes, including endoplasmic reticulum mannose trimming; positive regulation of retrograde protein transport, ER to cytosol; and protein targeting to ER. Predicted to act upstream of or within ERAD pathway.
Gene details: NCBI Gene ID: 297504; Chromosome location: 4q41
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.