SYBR Green qPCR primers for detecting rat Duox1 (dual oxidase 1) gene expression by real-time PCR.
Rat Duox1 qPCR Primer Set – dual oxidase 1
About this gene: Duox1 is predicted to enable multiple functions, including heme binding activity; nucleotide binding activity; and oxidoreductase activity, acting on NADH, oxygen as acceptor. It is involved in hydrogen peroxide biosynthetic process. Predicted to be active in plasma membrane.
Gene details: NCBI Gene ID: 266807; UniProt: Q8CIY2; Chromosome location: 3q35
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.