SYBR Green qPCR primers for detecting rat Dnmt1 (DNA methyltransferase 1) gene expression by real-time PCR.
Rat Dnmt1 qPCR Primer Set – DNA methyltransferase 1
About this gene: Dnmt1 enables multiple functions, including DNA-methyltransferase activity; histone deacetylase binding activity; and nuclear estrogen receptor binding activity. It is involved in multiple processes, including cellular response to lead ion; negative regulation of gene expression via chromosomal CpG island methylation; and positive regulation of macrophage fusion.
Gene details: NCBI Gene ID: 84350; Chromosome location: 8q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.