SYBR Green qPCR primers for detecting rat Dnai1 (dynein, axonemal, intermediate chain 1) gene expression by real-time PCR.
Rat Dnai1 qPCR Primer Set – dynein, axonemal, intermediate chain 1
Also known as: Dnaic1
About this gene: Dnai1 is predicted to enable dynein heavy chain binding activity and dynein light chain binding activity. It is predicted to be involved in cilium movement; insulin receptor signaling pathway; and outer dynein arm assembly. It is predicted to act upstream of or within multiple processes, including determination of left/right symmetry; epithelial cilium movement involved in extracellular fluid movement; and flagellated sperm motility.
Gene details: NCBI Gene ID: 500442; UniProt: Q5XIL8; Chromosome location: 5q22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.