SYBR Green qPCR primers for detecting rat Abat (4-aminobutyrate aminotransferase, also called GABA-T) gene expression by real-time PCR.
Rat Abat qPCR Primer Set – 4-aminobutyrate aminotransferase
Also known as: Gabat, beta-AlaAT
About this gene: Enables-3-amino-2-methylpropionate:2-oxoglutarate transaminase activity and 4-aminobutyrate:2-oxoglutarate transaminase activity. It is involved in multiple processes, including GABA biosynthetic process; positive regulation of inhibitory postsynaptic potential; and regulation of secretion.
Gene details: NCBI Gene ID: 81632; UniProt: P50554; Chromosome location: 10q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.