SYBR Green qPCR primers for detecting rat Dicer1 (dicer 1 ribonuclease III) gene expression by real-time PCR.
Rat Dicer1 qPCR Primer Set – dicer 1 ribonuclease III
About this gene: Dicer1 is predicted to enable multiple functions, including double-stranded RNA binding activity; endonuclease activity; and regulatory RNA binding activity. Predicted to contribute to pre-miRNA binding activity. It is involved in multiple processes, including positive regulation of cell population proliferation; positive regulation of collagen biosynthetic process; and positive regulation of endothelial cell-matrix adhesion.
Gene details: NCBI Gene ID: 299284; Chromosome location: 6q32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.