SYBR Green qPCR primers for detecting rat Dhcr24 (24-dehydrocholesterol reductase) gene expression by real-time PCR.
Rat Dhcr24 qPCR Primer Set – 24-dehydrocholesterol reductase
About this gene: Dhcr24 is predicted to enable enzyme binding activity; oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; and peptide antigen binding activity. It is involved in multiple processes, including Ras protein signal transduction; negative regulation of cell population proliferation; and steroid metabolic process.
Gene details: NCBI Gene ID: 298298; UniProt: Q5BQE6; Chromosome location: 5q34
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.