SYBR Green qPCR primers for detecting rat Dgat1 (diacylglycerol O-acyltransferase 1, also called ARAT) gene expression by real-time PCR.
Rat Dgat1 qPCR Primer Set – diacylglycerol O-acyltransferase 1
Also known as: ARAT, Dgat
About this gene: Dgat1 enables diacylglycerol O-acyltransferase activity; diacylglycerol binding activity; and fatty acid binding activity. It is involved in multiple processes, including insulin secretion; positive regulation of hepatic stellate cell activation; and regulation of lipid metabolic process. Predicted to be active in endoplasmic reticulum membrane.
Gene details: NCBI Gene ID: 84497; UniProt: Q9ERM3; Chromosome location: 7q34
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.