SYBR Green qPCR primers for detecting rat Decr1 (2,4-dienoyl-CoA reductase 1) gene expression by real-time PCR.
Rat Decr1 qPCR Primer Set – 2,4-dienoyl-CoA reductase 1
About this gene: Decr1 is predicted to enable 2,4-dienoyl-CoA reductase activity; NADPH binding activity; and identical protein binding activity. It is predicted to be involved in fatty acid beta-oxidation and positive regulation of cold-induced thermogenesis. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 117543; UniProt: Q64591; Chromosome location: 5q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.