SYBR Green qPCR primers for detecting rat Ddx4 (DEAD-box helicase 4, also called rVLG) gene expression by real-time PCR.
Rat Ddx4 qPCR Primer Set – DEAD-box helicase 4
Also known as: RVLG
About this gene: Ddx4 is predicted to enable multiple functions, including ATP hydrolysis activity; RNA helicase activity; and molecular condensate scaffold activity. It is involved in response to benzoic acid; response to retinoic acid; and spermatogenesis.
Gene details: NCBI Gene ID: 310090; UniProt: Q64060; Chromosome location: 2q14
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.