SYBR Green qPCR primers for detecting rat Ddx1 (DEAD-box helicase 1) gene expression by real-time PCR.
Rat Ddx1 qPCR Primer Set – DEAD-box helicase 1
About this gene: Ddx1 is predicted to enable multiple functions, including RNA binding activity; helicase activity; and transcription coregulator activity. It is predicted to be involved in multiple processes, including positive regulation of canonical NF-kappaB signal transduction; positive regulation of myeloid dendritic cell cytokine production; and protein localization to cytoplasmic stress granule. It is predicted to act upstream of or within response to exogenous dsRNA and response to virus.
Gene details: NCBI Gene ID: 84474; UniProt: Q641Y8; Chromosome location: 6q15
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.