SYBR Green qPCR primers for detecting rat Ddt (D-dopachrome tautomerase) gene expression by real-time PCR.
Rat Ddt qPCR Primer Set – D-dopachrome tautomerase
About this gene: Ddt enables D-dopachrome decarboxylase activity. It is predicted to be involved in multiple processes, including negative regulation of macrophage chemotaxis; positive regulation of ERK1 and ERK2 cascade; and positive regulation of tumor necrosis factor production. Predicted to be active in extracellular region.
Gene details: NCBI Gene ID: 29318; UniProt: P80254; Chromosome location: 20p12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.