SYBR Green qPCR primers for detecting rat Ddost (dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit) gene expression by real-time PCR.
Rat Ddost qPCR Primer Set – dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit
About this gene: Ddost is predicted to enable enzyme activator activity. It is predicted to be involved in multiple processes, including T cell activation; protein N-linked glycosylation; and regulation of protein stability.
Gene details: NCBI Gene ID: 313648; UniProt: Q641Y0; Chromosome location: 5q36
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.