SYBR Green qPCR primers for detecting rat Ddc (dopa decarboxylase, also called AADC) gene expression by real-time PCR.
Rat Ddc qPCR Primer Set – dopa decarboxylase
Also known as: AADC
About this gene: Ddc enables multiple functions, including amino acid binding activity; aromatic-L-amino-acid decarboxylase activity; and pyridoxal phosphate binding activity. It is involved in multiple processes, including aminergic neurotransmitter loading into synaptic vesicle; dopamine biosynthetic process; and response to pyrethroid.
Gene details: NCBI Gene ID: 24311; UniProt: P14173; Chromosome location: 14q21
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.