SYBR Green qPCR primers for detecting rat Dbt (dihydrolipoamide branched chain transacylase E2) gene expression by real-time PCR.
Rat Dbt qPCR Primer Set – dihydrolipoamide branched chain transacylase E2
About this gene: Dbt is predicted to enable ubiquitin protein ligase binding activity. It is involved in branched-chain amino acid catabolic process. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 29611; Chromosome location: 2q42
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.