SYBR Green qPCR primers for detecting rat Dazl (deleted in azoospermia-like) gene expression by real-time PCR.
Rat Dazl qPCR Primer Set – deleted in azoospermia-like
About this gene: Dazl is predicted to enable identical protein binding activity; mRNA 3'-UTR binding activity; and translation activator activity. It is predicted to be involved in 3'-UTR-mediated mRNA stabilization and positive regulation of translational initiation. It is predicted to act upstream of or within female meiosis II; oocyte maturation; and positive regulation of meiotic nuclear division.
Gene details: NCBI Gene ID: 680486; Chromosome location: 9q12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.