SYBR Green qPCR primers for detecting rat Dact1 (dishevelled-binding antagonist of beta-catenin 1) gene expression by real-time PCR.
Rat Dact1 qPCR Primer Set – dishevelled-binding antagonist of beta-catenin 1
Also known as: RGD1564008
About this gene: Dact1 is predicted to enable multiple functions, including beta-catenin binding activity; delta-catenin binding activity; and enzyme binding activity. It is predicted to be involved in multiple processes, including negative regulation of JNK cascade; negative regulation of beta-catenin-TCF complex assembly; and regulation of Wnt signaling pathway. It is predicted to act upstream of or within multiple processes, including dendrite morphogenesis; negative regulation of Wnt signaling pathway; and positive regulation of fat cell differentiation.
Gene details: NCBI Gene ID: 500666; Chromosome location: 6q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.