SYBR Green qPCR primers for detecting rat Cyp46a1 (cytochrome P450, family 46, subfamily a, polypeptide 1) gene expression by real-time PCR.
Rat Cyp46a1 qPCR Primer Set – cytochrome P450, family 46, subfamily a, polypeptide 1
About this gene: Cyp46a1 is predicted to enable cholesterol 24-hydroxylase activity; heme binding activity; and steroid hydroxylase activity. It is predicted to be involved in regulation of long-term synaptic potentiation; steroid metabolic process; and xenobiotic metabolic process. Predicted to act upstream of with a negative effect on protein localization to membrane raft.
Gene details: NCBI Gene ID: 362782; Chromosome location: 6q32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.