SYBR Green qPCR primers for detecting rat Cyp24a1 (cytochrome P450, family 24, subfamily a, polypeptide 1) gene expression by real-time PCR.
Rat Cyp24a1 qPCR Primer Set – cytochrome P450, family 24, subfamily a, polypeptide 1
Also known as: 24-OHase, 24R-Ohase, Cyp24, P450-CC24, VITD12
About this gene: Cyp24a1 enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity; 25-hydroxycholecalciferol-24-hydroxylase activity; and vitamin D3 25-hydroxylase activity. It is involved in cellular response to vitamin D and vitamin D catabolic process.
Gene details: NCBI Gene ID: 25279; UniProt: Q09128; Chromosome location: 3q42
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.