SYBR Green qPCR primers for detecting rat Cyp11a1 (cytochrome P450, family 11, subfamily a, polypeptide 1, also called CYPXIA1) gene expression by real-time PCR.
Rat Cyp11a1 qPCR Primer Set – cytochrome P450, family 11, subfamily a, polypeptide 1
Also known as: Cyp11a, Cypxia1, P450(scc), P450scc
About this gene: Cyp11a1 is predicted to enable cholesterol monooxygenase activity and heme binding activity. It is involved in multiple processes, including Leydig cell differentiation; cellular response to cytokine stimulus; and nervous system development.
Gene details: NCBI Gene ID: 29680; UniProt: P14137; Chromosome location: 8q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.