SYBR Green qPCR primers for detecting rat Cyb5r1 (cytochrome b5 reductase 1, also called b5R.1) gene expression by real-time PCR.
Rat Cyb5r1 qPCR Primer Set – cytochrome b5 reductase 1
Also known as: Nqo3a2
About this gene: Cyb5r1 is predicted to enable FAD binding activity and cytochrome-b5 reductase activity, acting on NADH. Predicted to be located in endoplasmic reticulum membrane. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 304805; UniProt: Q5EB81; Chromosome location: 13q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.