SYBR Green qPCR primers for detecting rat Cyb561d2 (cytochrome b561 family, member D2) gene expression by real-time PCR.
Rat Cyb561d2 qPCR Primer Set – cytochrome b561 family, member D2
About this gene: Cyb561d2 is predicted to enable heme binding activity; transmembrane ascorbate ferrireductase activity; and transmembrane monodehydroascorbate reductase activity. It is predicted to be involved in ascorbate homeostasis. Predicted to be active in endoplasmic reticulum membrane.
Gene details: NCBI Gene ID: 363137; UniProt: Q641Y1; Chromosome location: 8q32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.