SYBR Green qPCR primers for detecting rat Cul2 (cullin 2) gene expression by real-time PCR.
Rat Cul2 qPCR Primer Set – cullin 2
About this gene: Cul2 is predicted to enable ubiquitin ligase complex scaffold activity and ubiquitin protein ligase binding activity. Predicted to contribute to ubiquitin-protein transferase activity. It is predicted to be involved in SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; and ubiquitin-dependent protein catabolic process via the C-end degron rule pathway.
Gene details: NCBI Gene ID: 361258; Chromosome location: 17q12.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.