SYBR Green qPCR primers for detecting rat Cst7 (cystatin F) gene expression by real-time PCR.
Rat Cst7 qPCR Primer Set – cystatin F
About this gene: Cst7 is predicted to enable cysteine-type endopeptidase inhibitor activity; protease binding activity; and protein homodimerization activity. It is predicted to be involved in lysosomal protein catabolic process; negative regulation of microglial cell activation; and positive regulation of myelination. Predicted to be active in multiple cellular components, including Golgi apparatus; late endosome; and lysosome.
Gene details: NCBI Gene ID: 296257; Chromosome location: 3q41
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.