SYBR Green qPCR primers for detecting rat Crkl (CRK like proto-oncogene, adaptor protein) gene expression by real-time PCR.
Rat Crkl qPCR Primer Set – CRK like proto-oncogene, adaptor protein
About this gene: Crkl is predicted to enable multiple functions, including identical protein binding activity; phosphotyrosine residue binding activity; and receptor tyrosine kinase binding activity. It is predicted to be involved in multiple processes, including positive regulation of substrate adhesion-dependent cell spreading; postsynaptic specialization assembly; and regulation of intracellular signal transduction. It is predicted to act upstream of or within multiple processes, including cell surface receptor signaling pathway; circulatory system development; and nervous system development.
Gene details: NCBI Gene ID: 287942; UniProt: Q5U2U2; Chromosome location: 11q23
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.