SYBR Green qPCR primers for detecting rat Crb1 (crumbs cell polarity complex component 1) gene expression by real-time PCR.
Rat Crb1 qPCR Primer Set – crumbs cell polarity complex component 1
About this gene: Crb1 is predicted to enable calcium ion binding activity. It is predicted to be involved in establishment or maintenance of epithelial cell apical/basal polarity and heterophilic cell-cell adhesion. It is predicted to act upstream of or within multiple processes, including detection of light stimulus involved in visual perception; eye morphogenesis; and neurogenesis.
Gene details: NCBI Gene ID: 304825; Chromosome location: 13q13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.