SYBR Green qPCR primers for detecting rat Coq6 (coenzyme Q6 monooxygenase) gene expression by real-time PCR.
Rat Coq6 qPCR Primer Set – coenzyme Q6 monooxygenase
About this gene: Coq6 is predicted to enable 2-methoxy-6-polyprenolphenol 4-hydroxylase activity; 4-hydroxy-3-all-trans-polyprenylbenzoate oxygenase activity; and oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH as one donor, and incorporation of one atom of oxygen. It is predicted to be involved in ubiquinone biosynthetic process. Predicted to be active in mitochondrion.
Gene details: NCBI Gene ID: 299195; UniProt: Q68FU7; Chromosome location: 6q31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.