SYBR Green qPCR primers for detecting rat Clgn (calmegin) gene expression by real-time PCR.
Rat Clgn qPCR Primer Set – calmegin
About this gene: Clgn is predicted to enable calcium ion binding activity and protein folding chaperone. It is predicted to be involved in ERAD pathway and protein folding. It is predicted to act upstream of or within binding activity of sperm to zona pellucida and protein-containing complex assembly.
Gene details: NCBI Gene ID: 685504; Chromosome location: 19q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.