SYBR Green qPCR primers for detecting rat Chst3 (carbohydrate sulfotransferase 3) gene expression by real-time PCR.
Rat Chst3 qPCR Primer Set – carbohydrate sulfotransferase 3
About this gene: Chst3 enables proteoglycan sulfotransferase activity. It is involved in chondroitin sulfate proteoglycan biosynthetic process and peripheral nervous system axon regeneration. Predicted to be active in Golgi apparatus.
Gene details: NCBI Gene ID: 84468; UniProt: Q9QZL2; Chromosome location: 20q11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.