SYBR Green qPCR primers for detecting rat Chrnb3 (cholinergic receptor nicotinic beta 3 subunit) gene expression by real-time PCR.
Rat Chrnb3 qPCR Primer Set – cholinergic receptor nicotinic beta 3 subunit
About this gene: Chrnb3 enables acetylcholine binding activity. Contributes to heterocyclic compound binding activity. It is predicted to be involved in multiple processes, including presynaptic modulation of chemical synaptic transmission; response to nicotine; and synaptic signaling.
Gene details: NCBI Gene ID: 171131; UniProt: P12391; Chromosome location: 16q12.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.