SYBR Green qPCR primers for detecting rat Chrm5 (cholinergic receptor, muscarinic 5) gene expression by real-time PCR.
Rat Chrm5 qPCR Primer Set – cholinergic receptor, muscarinic 5
About this gene: Chrm5 enables G protein-coupled acetylcholine receptor activity. It is involved in regulation of phosphatidylinositol dephosphorylation. Predicted to be active in dendrite; plasma membrane; and synapse.
Gene details: NCBI Gene ID: 53949; UniProt: P08911; Chromosome location: 3q35
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.