SYBR Green qPCR primers for detecting rat Chrm4 (cholinergic receptor, muscarinic 4) gene expression by real-time PCR.
Rat Chrm4 qPCR Primer Set – cholinergic receptor, muscarinic 4
Also known as: M4
About this gene: Chrm4 enables guanyl-nucleotide exchange factor activity. It is predicted to be involved in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; and chemical synaptic transmission. Is active in glutamatergic synapse; postsynaptic density membrane; and presynaptic membrane.
Gene details: NCBI Gene ID: 25111; UniProt: P08485; Chromosome location: 3q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.