SYBR Green qPCR primers for detecting mouse Atp5pf (ATP synthase peripheral stalk subunit F6) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Atp5pf qPCR Primer Set – ATP synthase peripheral stalk subunit F6
Also known as: Atp5J, Atp5j, CF6
About this gene: The Atp5pf protein is a component of mitochondrial adenosine triphosphate synthase, which catalyzes the conversion of ATP from ADP. Mitochondrial adenosine triphosphate synthase consists of extrinsic and intrinsic membrane domains that are joined by a stalk. The Atp5pf protein is a subunit of the stalk domain. A bi-directional promoter that drives expression of Atp5pf has been has been identified.
Gene details: NCBI Gene ID: 11957; UniProt: P97450; Chromosome location: 16 C3.3|16 46.92 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
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