SYBR Green qPCR primers for detecting mouse Asah1 (N-acylsphingosine amidohydrolase 1, also called AC) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Asah1 qPCR Primer Set – N-acylsphingosine amidohydrolase 1
Also known as: 2310081N20Rik, AC, Asah
About this gene: Asah1 encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The Asah1 protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of Asah1 leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype.
Gene details: NCBI Gene ID: 11886; UniProt: Q9WV54; Chromosome location: 8 A4|8 23.89 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.