SYBR Green qPCR primers for detecting mouse Arfrp1 (ARF related protein 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Arfrp1 qPCR Primer Set – ARF related protein 1
Also known as: 1500006I01Rik
About this gene: The gene encodes a membrane-associated GTPase that is related to the ADP-ribosylation factor and ARF-like genes. The Arfrp1 gene product plays an essential role in Golgi function controlling recruitment of GRIP domain proteins and ARL1 to the trans-Golgi and trans-Golgi to plasma membrane trafficking of cell surface proteins such as E-cadherin. Deletion of Arfrp1 in mice leads to embryonic lethality during early gastrulation, which is at least partly caused by the disruption of E-cadherin trafficking to the cell surface and therefore lack of sufficient cell-cell adhesion in the embryo.
Gene details: NCBI Gene ID: 76688; UniProt: Q8BXL7; Chromosome location: 2 H4|2 103.63 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.