SYBR Green qPCR primers for detecting mouse Alpl (alkaline phosphatase, liver/bone/kidney, also called TNAP) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Alpl qPCR Primer Set – alkaline phosphatase, liver/bone/kidney
Also known as: ALP, APTNAP, Akp-2, Akp2, TNAP, TNSALP
About this gene: Alpl encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The Alpl protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones.
Gene details: NCBI Gene ID: 11647; UniProt: P09242; Chromosome location: 4 D3|4 70.02 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.