SYBR Green qPCR primers for detecting human TIGAR (TP53 induced glycolysis regulatory phosphatase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human TIGAR qPCR Primer Set – TP53 induced glycolysis regulatory phosphatase
Also known as: C12orf5, FR2BP
About this gene: TIGAR is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis.
Gene details: NCBI Gene ID: 57103; HGNC ID: HGNC:1185; UniProt: Q9NQ88; RefSeq (MANE Select): NM_020375.3; Chromosome location: 12p13.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.