SYBR Green qPCR primers for detecting human ACO1 (aconitase 1, also called IRP1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ACO1 qPCR Primer Set – aconitase 1
Also known as: ACONS, HEL60, IREB1, IREBP, IREBP1, IRP1
About this gene: The ACO1 protein is a bifunctional, cytosolic protein that acts as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and acts as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate.
Gene details: NCBI Gene ID: 48; HGNC ID: HGNC:117; UniProt: P21399; RefSeq (MANE Select): NM_002197.3; Chromosome location: 9p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.