SYBR Green qPCR primers for detecting human AAK1 (AP2 associated kinase 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human AAK1 qPCR Primer Set – AP2 associated kinase 1
About this gene: AAK1 belongs to the SNF1 subfamily of serine/threonine protein kinases. Adaptor-related protein complex 2 functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. The AAK1 protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis.
Gene details: NCBI Gene ID: 22848; HGNC ID: HGNC:19679; UniProt: Q2M2I8; RefSeq (MANE Select): NM_014911.5; Chromosome location: 2p13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.