SYBR Green qPCR primers for detecting human BLM (BLM RecQ like helicase, also called RecQ2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human BLM qPCR Primer Set – BLM RecQ like helicase
Also known as: BS, MGRISCE1, RECQ2, RECQL2, RECQL3
About this gene: The Bloom syndrome is an autosomal recessive disorder characterized by growth deficiency, microcephaly and immunodeficiency among others. The BLM gene product is caused by homozygous or compound heterozygous mutation in the gene encoding DNA helicase RecQ protein on chromosome 15q26. This Bloom-associated helicase unwinds a variety of DNA substrates including Holliday junction, and is involved in multiple pathways contributing to the maintenance of genome stability.
Gene details: NCBI Gene ID: 641; HGNC ID: HGNC:1058; UniProt: P54132; RefSeq (MANE Select): NM_000057.4; Chromosome location: 15q26.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.