SYBR Green qPCR primers for detecting human AADAT (aminoadipate aminotransferase, also called KAT/AadAT) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human AADAT qPCR Primer Set – aminoadipate aminotransferase
Also known as: KAT2, KATII, KYAT2
About this gene: AADAT encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties.
Gene details: NCBI Gene ID: 51166; HGNC ID: HGNC:17929; UniProt: Q8N5Z0; RefSeq (MANE Select): NM_016228.4; Chromosome location: 4q33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.