SYBR Green qPCR primers for detecting human BCKDHB (branched chain keto acid dehydrogenase E1 subunit beta, also called BCKDE1B) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human BCKDHB qPCR Primer Set – branched chain keto acid dehydrogenase E1 subunit beta
Also known as: BCKDE1B, BCKDH E1-beta, E1B, MSUD1B, OVD1B
About this gene: BCKDHB encodes a E1 beta subunit of branched-chain keto acid dehydrogenase, which is a multienzyme complex linked to the inner membrane of mitochondria. This enzyme complex functions in the catabolism of branched-chain amino acids. Mutations in BCKDHB have been linked to maple syrup urine disease, type 1B, a disease characterized by a maple syrup odor to the urine also to mental and physical retardation and feeding problems.
Gene details: NCBI Gene ID: 594; HGNC ID: HGNC:987; UniProt: P21953; RefSeq (MANE Select): NM_183050.4; Chromosome location: 6q14.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.