SYBR Green qPCR primers for detecting human BARD1 (BRCA1 associated RING domain 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human BARD1 qPCR Primer Set – BRCA1 associated RING domain 1
About this gene: BARD1 encodes a protein which interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, it shares homology with the 2 most conserved regions of BRCA1: the N-terminal RING motif and the C-terminal BRCT domain. The RING motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes.
Gene details: NCBI Gene ID: 580; HGNC ID: HGNC:952; UniProt: Q99728; RefSeq (MANE Select): NM_000465.4; Chromosome location: 2q35
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.