SYBR Green qPCR primers for detecting human ACADM (acyl-CoA dehydrogenase medium chain, also called MCAD) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ACADM qPCR Primer Set – acyl-CoA dehydrogenase medium chain
Also known as: ACAD1, MCAD, MCADH
About this gene: ACADM encodes a medium-chain specific acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in ACADM cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death.
Gene details: NCBI Gene ID: 34; HGNC ID: HGNC:89; UniProt: P11310; RefSeq (MANE Select): NM_000016.6; Chromosome location: 1p31.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.