SYBR Green qPCR primers for detecting human AUH (AU RNA binding methylglutaconyl-CoA hydratase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human AUH qPCR Primer Set – AU RNA binding methylglutaconyl-CoA hydratase
About this gene: AUH encodes bifunctional mitochondrial protein that has both RNA-binding and hydratase activities. The AUH protein is a methylglutaconyl-CoA hydratase that catalyzes the hydration of 3-methylglutaconyl-CoA to 3-hydroxy-3-methyl-glutaryl-CoA, a critical step in the leucine degradation pathway. The AUH protein also binds AU-rich elements found in the 3' UTRs of rapidly decaying mRNAs including c-fos, c-myc and granulocyte/ macrophage colony stimulating factor.
Gene details: NCBI Gene ID: 549; HGNC ID: HGNC:890; UniProt: Q13825; RefSeq (MANE Select): NM_001698.3; Chromosome location: 9q22.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.