SYBR Green qPCR primers for detecting human ATP5MF (ATP synthase membrane subunit f) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ATP5MF qPCR Primer Set – ATP synthase membrane subunit f
Also known as: ATP5J2, ATP5JL
About this gene: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The ATP5MF gene product is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The catalytic portion of mitochondrial ATP synthase consists of five different subunits assembled with a stoichiometry of 3 alpha, 3 beta, and single representatives of the gamma, delta, and epsilon subunits.
Gene details: NCBI Gene ID: 9551; HGNC ID: HGNC:848; UniProt: P56134; RefSeq (MANE Select): NM_004889.5; Chromosome location: 7q22.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.