SYBR Green qPCR primers for detecting human ATP5PF (ATP synthase peripheral stalk subunit F6) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ATP5PF qPCR Primer Set – ATP synthase peripheral stalk subunit F6
Also known as: ATP5, ATP5A, ATP5J, ATPM, CF6, F6
About this gene: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. The ATP5PF gene product is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3.
Gene details: NCBI Gene ID: 522; HGNC ID: HGNC:847; UniProt: P18859; RefSeq (MANE Select): NM_001003703.2; Chromosome location: 21q21.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.