SYBR Green qPCR primers for detecting human ATP4A (ATPase H+/K+ transporting subunit alpha) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human ATP4A qPCR Primer Set – ATPase H+/K+ transporting subunit alpha
Also known as: ATP6A
About this gene: The ATP4A protein belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H and K ions across the plasma membrane.
Gene details: NCBI Gene ID: 495; HGNC ID: HGNC:819; UniProt: P20648; RefSeq (MANE Select): NM_000704.3; Chromosome location: 19q13.12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.